NF-κB activation is responsible for the synergistic effect of herpes simplex virus type 2 infection on interferon-γ-induced nitric oxide production in macrophages
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چکیده
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منابع مشابه
Kinetics of Nitric Oxide Production and MTT Reduction by HSV-1 Infected Macrophages
Background: Macrophages have important role in defense against Herpes Simplex Virus type-1 (HSV-1). The present study was performed to determine the viability and nitric oxide (NO) production by HSV-1 infected mouse peritoneal macrophages (HIM). Method: The viability of macrophages was evaluated using MTT reduction assay and the production of nitrite using Griess method. Results: The ability of...
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Objective(s) Previous studies have demonstrated that the nitric oxide (NO) dependent death of murine peritoneal macrophages activated in vitro with IFN-g and LPS is mediated through apoptosis. In the present study, we investigated the synergistic effect of LPS, IFN-g and iron on NO production and apoptosis. Materials and Methods After determination of iron cytotoxicity, the peritoneal macrop...
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OBJECTIVE T cell immunoglobulin- and mucin-domain-containing molecule-4 (Tim-4) receives much attention as a potentially negative regulator of immune responses. However, its modulation on macrophages has not been fully elucidated so far. This study aimed to identify the role of Tim-4 in nitric oxide (NO) modulation. METHODS Macrophages were stimulated with 100 ng/ml LPS or 100 U/ml IFN-γ. RT-...
متن کاملHerpes simplex virus type 2 induces secretion of IL-12 by macrophages through a mechanism involving NF-kappaB.
Interleukin (IL)-12 is an important proinflammatory and immunoregulatory cytokine expressed primarily by macrophages. Although IL-12 appears to be essential for clearance of many bacterial and parasitic infections, only little is known about the production and regulation of this cytokine during viral infections. In this study we have shown that infection of mouse macrophages with herpes simplex...
متن کاملEffect of Neutrophils on Nitric Oxide Production from Stimulated Macrophages
Background: During the initial phase of an infection, there is an upregulation of inducible nitric oxide synthase in the macrophages for the production of nitric oxide. This is followed by the recruitment of polymorphonuclear leukocytes (neutrophils) which release arginase. Arginase competes with inducible nitric oxide synthase for a common substrate L-arginine. Objective: To investigate whethe...
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تاریخ انتشار 1998